Coding

Part:BBa_K2729010:Design

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)


DENV1 ancC-prM-E (Dengue Virus Serotype I)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1245
    Illegal BglII site found at 1856
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1831
    Illegal AgeI site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217
    Illegal BsaI.rc site found at 367


Design Notes

Since the genes are separated from non-structural region, the proteins and the replicated genes are non-infectious and not reproduced after the first infection.


Source

Since the genes consist of dengue virus structural region, they are derived from dengue virus.

References